美國Orbigen
Orbigen位于風景如畫的美國加州SAN DIEGO,其主要成員來自該地區著名的生物科技公司和大學,在真核表達,逆轉錄表達,SiRNA,抗體開發等方面居于世界領先地位。著名品牌有:
Sapphire Baculovirus真核表達系統及預制基因庫
Orbigen公司可以提供幾百種重組的桿狀病毒表達載體,其涉及的外源基因主要來自于人、大鼠、小鼠、酵母、病毒等多種生物。除提供插入單個外源基因的重組桿狀病毒表達載體外,該公司還可以提供多種插入GST、His6標記融合基因的重組桿狀病毒表達載體,極大地方便了客戶的科研工作。
Phoenix Retrovirus逆轉錄表達系統
該系統應用非常廣泛,目前世界上有近2500家實驗室采用該系統轉染將外源基因轉入分化的哺乳動物細胞。采用LacZ和GFP基因作為報告系統。
試劑盒組分:
* PhoenixTM ampho 包裝細胞
* PhoenixTM eco 包裝細胞
* 10 μg pBMNZ 質粒
* 10 μg pBMN-GFP 質粒
BaculoBooster Virus Kit
該試劑盒包括10種不同的桿狀病毒庫,每個桿狀病毒庫都含有表達一組真核生物伴侶蛋白的重組桿狀病毒混合物,這些伴侶蛋白可以在感染的昆蟲細胞(Sf9, Sf21 and T.ni)知道重組蛋白的折疊。該桿狀病毒庫可以與感染細胞內的重組桿狀病毒相互作用,優化目的蛋白的表達水平和折疊。
pCMV1/2/3 Xi-Clone 和pIX1/2/3 Xi-Clone 克隆表達系統
pCMV1/2/3 Xi-Clone?系統有3種不同的類型,每種類型分別包含一種pCMV載體,可以快速高效地實現體內定向克隆。這類pCMV載體含有高度優化的細胞巨化病毒(CMV)的啟動子/增強子序列,因此表達效率比其它的CMV類載體(如:pcDNA3)要高好幾倍。
pIX1/2/3 Xi-Clone?含有有3種不同的類型(pIX、pIX2和pIX3),每種類型分別包含一種載體,可以快速構建質粒模板,實現體外的快速轉錄和翻譯。該類優化的載體含有T7啟動子,采用Xi-Clone定向克隆技術。
特點:
* 3種不同的配置選擇:不標記表達、N-端HA融合標記和C-端HA融合標記 。采用G418選擇穩定轉染的細胞
* 利用anti-HA抗體可以快速檢測表達蛋白
Orbigen develops, manufactures and markets reagent tools for proteomic research. We are best known for novel antibodies, innovative gene expression technologies, and reliable custom protein expression services. By being at the forefront of technology development, we are proud to provide timely and quality products that help you continuously push the limits of what's possible.
Orbigen is a young and rapidly growing company headquartered in San Diego, California, one of the world's largest clusters of life science research institutes and companies. We employ an extensive group of dynamic and talented scientists who are empowered to create unique and exciting solutions to address your research needs. Many of our scientists are founding members and seasoned veterans from industry leaders. Our experienced technical staff strives to provide high-quality products and expert technical support to life science researchers worldwide.
In addition to providing unique research tools we can also help you commercialize your ideas and reagents. We have extensive experience and proven track record for working with inventors to timely and effectively bring important reagent to market.